1. The construction and operation of the canal will change the hydrological balance of the delta - it will enhance the run-off in Bystroe estuary and reduce the waterflow for other estuaries - which will lead to harmful consequences both for natural ecosystems and the water supply regime in the town of Vilkovo .
2. Canal operation will lead to oil and oil product pollution of the estuary.
3. The construction works and operation of the canal will cause damage to the habitat and feeding base of the majority of the fish species dwelling in this area, including 7 species which are listed in the European Red List and the 16 listed in the Ukraine Red Book. Also, as the result of damage to feeding grounds, the annual freshwater fish catch will go down by 19 tons, and the marine fish catch - by 80 tons.
4. The spawning migration of the Danube herring (Alosa pontica (Eichwald) passes through the Bystroye Estuary. The construction and operation of the canal will destroy the natural course of breeding of this fish that will reduce its industrial catch by 90 tons, and will also threaten its existence.
5. The waves caused by ships passing through the canal will lead to the death of young fish, including the danube herring.
6. Deepening of the canal bottom and permanent works to sustain its condition will lead to the demise of young valuable trade and rare fish: Acipenseridae, Gymnocephalus schraetser, Zingel zingel and Z. streber streber.
7. Construction of embankments on the banks of the Bystroye Estuary will spoil the spawning conditions of many fish species.
8. The sandbank in front of the Bystroye is the foraging area of many commercially fished species. The construction and operation of the canal, and also the subsequent washing away of the sandbank will destroy the feeding conditions.
9. In the Danube Biosphere Reserve, 257 kinds of birds are present, 9 of them are on European Red List, 42 of them are listed in the Red Book of Ukraine. It is a habitat for thousands of wetland-dwelling birds. It is nesting ground for 1000 couples of Phalacrocorax pygamaeus, 3 couples of Haliaeetus albicilla. During the seasonal accumulation upto 2.500 Pelecanus onocrotalus and up to 80 Pelecanus crispus are found. Up to 800 Phalacrocorax pygamaeus, up to 900 Rufibrenta ruficollis and up to 35 of Haliaeetus albicilla are wintering within the Ukrainian part of the Danube reserve. Withing the direct impact zone of the ship course, mainly sandbank area, 223 bird species are found, 5 of them from European Red list, 31 from the Red Book of Ukraine. The construction and operation of the canal will result in losses to their mass nesting area; breeding conditions of the birds will be worsened, and the places of rest, feed and wintering will be lost. Thus, the construction of the canal through the Bystroye Estuary threatens the very existence of "The Danube Delta", part of the Ramsar Reserve in Ukraine.
10. The reserve vascular plant community encounters 950 species, 3 of which are on the European Red List, 16 - in the Red Book of Ukraine. Within the proposed canal construction area half of the species is growing, there are almost no immigrant species. The plaint community of the reserve is significantly different from plat communties of other deltas of north-west Black Sea coast. It has majorly water and wetland communities inhabiting up to 80% fo the area. The construction work impact on the plant community will be two-sided: the ship waves impact will cause to change in plant communities - many species, including rare, will vanish. On the other hand, permanent ship communication will bring invasive species (some of them quarantine) in. Changes in hidrological regime of Kilian Delta of Danube will lead to speeded euthrophication of the inner water ponds, disappearance of small estuaries, general turning of wetlands into meadows. This will also negatively influence one of the most important ecological functions of the Danube Delta - biofiltration.
11. The proposed canal will break the core zone of the biosphere reserve and will result in increased noise pollution in 5 km zone around canal, and negative influences on the fauna of the reserve. The reserve territory is a constant or temporary habitat for rare and endangered insects, amphibia and reptiles, many of which are on European red list or in the Red book of the Ukraine. The habitation of the majority of mentioned vulnerable species are intolerant to any similar activity.

The livelihoods in the area near the mouth of the Danube are based mainly on fishery. The construction of the canal threatens to undermine this branch of trade, and may destroy the traditional system of management in the region. Therefore, not only scientific and environmental organizations, but also local residents and authorities oppose building the canal through Bystroye.

Construction of the navigation canal "the Danube - the Black Sea" through Bystroye Estuary will cause degradation of natural communities in the Danube Delta, as well as destroy traditional forms of nature management, and can result in the worsening of social and economic conditions in the region. In other words, realization of this project will violate the principles of management of biosphere reserves defined by UNESCO in its "The Man and Biosphere" Program.

And as a result, the building of the canal through the Bystroye Estuary threatens the very existence of Danube Biosphere Reserve and Ukrainian Ramsar lands "The Danube Delta".